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Anti-GPR56 antibody [EPR23908-115] (ab274419)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-GPR56 antibody [EPR23908-115] (ab274419)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23908-115] to GPR56
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-GPR56 antibody [EPR23908-115]
    See all GPR56 primary antibodies
  • Description

    Rabbit monoclonal [EPR23908-115] to GPR56
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain, hippocampus and glioma tissue lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23908-115
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Neurology process
    • Neurogenesis

Images

  • Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)
    Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)
    Anti-GPR56 antibody [EPR23908-115] (ab274419) at 1/1000 dilution + Human glioma tissue lysate at 10 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17932623).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 70 seconds.

     

  • Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)
    Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)
    All lanes : Anti-GPR56 antibody [EPR23908-115] (ab274419) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human hippocampus tissue lysate
    Lane 3 : Human lung tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17932623).

    Negative control: Human lung (PMID: 15674329)

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Anti-GPR56 antibody [EPR23908-115] (ab274419)
    Anti-GPR56 antibody [EPR23908-115] (ab274419)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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