Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)]
    See all TFIIE alpha/GTF2E1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TFIIE alpha/GTF2E1 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Molt-4, HeLa, Jurkat and A431 cell lysates. Lung carcinoma tissue.

  • General notes

     

     

     This product was previously labelled as TFIIE alpha

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    Western blot - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    All lanes : Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TFIIE alpha/GTF2E1 with ab140634 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)

    Immunohistochemical analysis of paraffin embedded Normal Human Testis tissue using ab140634 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)

    Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140634 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)

    Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab140634 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)

    Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140634 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)
    Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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