Anti-TFIIE alpha/GTF2E1 antibody (ab28177)
Key features and details
- Rabbit polyclonal to TFIIE alpha/GTF2E1
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFIIE alpha/GTF2E1 antibody
See all TFIIE alpha/GTF2E1 primary antibodies -
Description
Rabbit polyclonal to TFIIE alpha/GTF2E1 -
Host species
Rabbit -
Specificity
ab28177 recognises TFIIE alpha/GTF2E1.
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Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
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Immunogen
Recombinant full length protein corresponding to Human TFIIE alpha/GTF2E1.
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Positive control
- HeLa nuclear extract
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General notes
This product was previously labelled as TFIIE alpha
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.9
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-TFIIE alpha/GTF2E1 antibody (ab28177)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
