Anti-GTF2E2 antibody (ab228581)
Key features and details
- Rabbit polyclonal to GTF2E2
- Suitable for: WB, IP, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GTF2E2 antibody
See all GTF2E2 primary antibodies -
Description
Rabbit polyclonal to GTF2E2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
-
Immunogen
Recombinant fragment within Human GTF2E2 (internal sequence). The exact sequence is proprietary.
Database link: P29084 -
Positive control
- ChIP: HeLa chromatin extract. IP: Jurkat whole cell lysate/extract. WB: Jurkat nuclear lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-GTF2E2 antibody (ab228581)Anti-GTF2E2 antibody (ab228581) at 1/5000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) nuclear lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 33 kDa10% SDS-PAGE
-
Immunoprecipitation - Anti-GTF2E2 antibody (ab228581)GTF2E2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract using 2.5 µg of ab228581. Western blot was performed from the immunoprecipitate using ab228581 at 1/1000 dilution.
Lane 1: Jurkat whole cell extract 30 μg (Input).
Lane 1: Control IgG IP in Jurkat whole cell extract.
Lane 2: ab228581 IP in Jurkat whole cell extract.
12% SDS-PAGE
-
ChIP - Anti-GTF2E2 antibody (ab228581)Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228581. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
