Anti-SLPI antibody (ab82337)
Key features and details
- Rabbit polyclonal to SLPI
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLPI antibody
See all SLPI primary antibodies -
Description
Rabbit polyclonal to SLPI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SLPI aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab129950) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Lung; Human Spleen; Human Small Intestine; Human Colon. It also gave a positive signal in HeLa, Hek293 and HepG2 cells in IF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB 1/250. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
1/250. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).Target
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Function
Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues. -
Tissue specificity
Mucous fluids. -
Sequence similarities
Contains 2 WAP domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6590 Human
- GenBank: NM_003064 Human
- Omim: 107285 Human
- SwissProt: P03973 Human
- Unigene: 517070 Human
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Alternative names
- ALK 1 antibody
- ALK1 antibody
- ALP antibody
see all
Images
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Western blot - Anti-SLPI antibody (ab82337)All lanes : Anti-SLPI antibody (ab82337) at 1/250 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lane 4 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 29 kDa, 48 kDa, 8 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-SLPI antibody (ab82337)ICC/IF image of ab82337 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82337, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and HepG2 cells at 5µg/ml.
Protocols
Datasheets and documents
References (0)
ab82337 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-SLPI antibody (ab82337)All lanes : Anti-SLPI antibody (ab82337) at 1/250 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lane 4 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 29 kDa, 48 kDa, 8 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-SLPI antibody (ab82337)ICC/IF image of ab82337 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82337, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and HepG2 cells at 5µg/ml.
