Donkey Anti-Rabbit IgG H&L (ab182020)
Key features and details
- Donkey Anti-Rabbit IgG H&L
- Host species: Donkey
- Isotype: IgG
- Suitable for: ELISA, WB, IHC-P, IP
Overview
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Product name
Donkey Anti-Rabbit IgG H&L
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
The antibody reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY, less than 2%. -
Tested applications
Suitable for: ELISA, WB, IHC-P, IPmore details -
Immunogen
The details of the immunogen for this antibody are not available.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ELISA - Donkey Anti-Rabbit IgG H&L (ab182020)
Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.
