Anti-MnSOD antibody (ab232961)
Key features and details
- Rabbit polyclonal to MnSOD
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-MnSOD antibody
See all MnSOD primary antibodies -
Description
Rabbit polyclonal to MnSOD -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human MnSOD aa 1 to the C-terminus. Full length mature chain without transit peptide. Expressed in E.coli. N-terminal tags.
Database link: P04179-1 -
Positive control
- WB: Recombinant human MnSOD protein; Human serum; Pig liver and brain lysates; HeLa and MCF7 cell lysates. IHC-P: Human liver, prostate cancer, breast cancer, kidney, skin cancer and colorectal cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MnSOD antibody (ab232961)All lanes : Anti-MnSOD antibody (ab232961) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : Pig liver lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : Pig brain lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 25 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MnSOD antibody (ab232961)Formalin-fixed, paraffin-embedded human liver tissue stained for MnSOD using ab232961 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MnSOD antibody (ab232961)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for MnSOD using ab232961 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MnSOD antibody (ab232961)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for MnSOD using ab232961 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MnSOD antibody (ab232961)Formalin-fixed, paraffin-embedded human kidney tissue stained for MnSOD using ab232961 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MnSOD antibody (ab232961)Formalin-fixed, paraffin-embedded human skin cancer tissue stained for MnSOD using ab232961 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MnSOD antibody (ab232961)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for MnSOD using ab232961 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-MnSOD antibody (ab232961)Anti-MnSOD antibody (ab232961) at 2 µg/ml + Recombinant human MnSOD protein
Predicted band size: 25 kDa
