Anti-GTF2E2 antibody [EPR14929] (ab187143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14929] to GTF2E2
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GTF2E2 antibody [EPR14929]
See all GTF2E2 primary antibodies -
Description
Rabbit monoclonal [EPR14929] to GTF2E2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanIP HumanWB MouseHuman -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, Jurkat, 293T, mouse heart, rat heart and rat brain lysates; Human cervix carcinoma and mouse liver tissues; 293T cells.
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General notes
This product was previously labelled as TFIIE beta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14929 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/10000 dilution
Lane 1 : K562 lysate
Lane 2 : Jurkat lysate
Lane 3 : 293T lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.
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All lanes : Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/1000 dilution
Lanes 1 & 3 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation. ab187143 at 1/50 labeling GTF2E2 immunoprecipitated from Jurkat cell lysate using ab187143 at 1/30.
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Immunofluorescent analysis of 293T cells labeling GTF2E2 with ab187143 at 1/250 and DAPI staining (blue).
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Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling GTF2E2 with ab187143 at 1/250 with a prediluted HRP Polymer for Rabbit IgG as a secondary antibody.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue labeling GTF2E2 with ab187143 at 1/250 with prediluted HRP Polymer for Rabbit IgG as a secondary antibody.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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