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Signal Transduction Second Messenger Nucleotide Messenger GTP

Anti-PREX1 antibody [6F12] (ab264535)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-PREX1 antibody [6F12] (ab264535)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [6F12] to PREX1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-PREX1 antibody [6F12]
    See all PREX1 primary antibodies
  • Description

    Mouse monoclonal [6F12] to PREX1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T47D, RAW264.7 and PC-12 whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    6F12
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-PREX1 antibody [6F12] (ab264535)
    Western blot - Anti-PREX1 antibody [6F12] (ab264535)
    All lanes : Anti-PREX1 antibody [6F12] (ab264535) at 1/1000 dilution

    Lane 1 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 186 kDa
    Observed band size: 186 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MDA-MB-231 (PMID: 21172654).

    Exposure time: 59 seconds.

  • Western blot - Anti-PREX1 antibody [6F12] (ab264535)
    Western blot - Anti-PREX1 antibody [6F12] (ab264535)
    All lanes : Anti-PREX1 antibody [6F12] (ab264535) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 186 kDa
    Observed band size: 186 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Anti-PREX1 antibody [6F12] (ab264535)
    Anti-PREX1 antibody [6F12] (ab264535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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