Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
-
Product name
Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free
See all TFIIE alpha/GTF2E1 primary antibodies -
Description
Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab248917 is the carrier-free version of ab140634.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8377(2) -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634) at 1/1000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDaThis data was developed using ab140634, the same antibody clone in a different buffer formulation.
-
This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TFIIE alpha/GTF2E1 with ab140634 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Normal Human Testis tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -