Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal (ab210924)
![Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal (ab210924)](https://www.abcam.com/ps/products/210/ab210924/Images/ab210924-266847-anti-nfkb-p105-p50-antibody-abm20h5-n-terminal-western-blot.jpg "Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal (ab210924)")
Key features and details
- Mouse monoclonal [ABM20H5] to NFkB p105 / p50 - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal
See all NFkB p105 / p50 primary antibodies -
Description
Mouse monoclonal [ABM20H5] to NFkB p105 / p50 - N-terminal -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human NFkB p105/ p50 aa 1-200 (N terminal).
Database link: P19838 -
Positive control
- THP1, Ramos, Jurkat and Raji lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM20H5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal (ab210924)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: NFκB p105/p50 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab210924 observed at 50 and 120 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab210924 was shown to specifically react with NFκB p105/p50 when NFκB p105/p50 knockout samples were used. Wild-type and NFκB p105/p50 knockout samples were subjected to SDS-PAGE. ab210924 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal (ab210924)](https://www.abcam.com/ps/products/210/ab210924/Images/ab210924-258425-anti-nfkb-p105-p50-antibody-abm20h5-n-terminal-western-blot.jpg)
Western blot - Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal (ab210924)All lanes : Anti-NFkB p105 / p50 antibody [ABM20H5] - N-terminal (ab210924) at 1 µg/ml
Lane 1 : THP1 lysate
Lane 2 : Ramos lysate
Lane 3 : Jurkat lysate
Lane 4 : Raji lysate
Predicted band size: 105 kDa
