Anti-Hsp60 antibody (ab31115)
Key features and details
- Rabbit polyclonal to Hsp60
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Hsp60 antibody
See all Hsp60 primary antibodies -
Description
Rabbit polyclonal to Hsp60 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pufferfish, Zebrafish, Sea urchin, Chinese hamster
-
Immunogen
Synthetic peptide within Human Hsp60 aa 1-100. The exact sequence is proprietary.
Database link: P10809 -
Positive control
- Hela cells, NIH3T3 cells, Colon carcinoma.
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Hsp60 antibody (ab31115) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Hsp60 antibody (ab31115) at 1/8000 dilution
Lanes 1 & 5 : Cow normal mammary epithelial cells (nMEC) treated with serum free media
Lanes 2 & 6 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin
Lanes 3 & 7 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1
Lanes 4 & 8 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : ECL Anti-Rabbit IgG, Horseradish Peroxidase Linked at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 15 secondsTreatment for 30 minutes (lanes 1-4) or 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab31115)Ab31115, at a 1/200 dilution, staining Hsp60 in formalin fixed, paraffin embedded human colon carcinoma tissue by Immunohistochemistry. -
Western blot - Anti-Hsp60 antibody (ab31115)Anti-Hsp60 antibody (ab31115) at 1/25 dilution + lysate prepared from HeLa cells
Predicted band size: 60 kDa
