Anti-Parvalbumin antibody [3C9] (ab277625)
![Anti-Parvalbumin antibody [3C9] (ab277625)](https://www.abcam.com/ps/products/277/ab277625/Images/ab277625-432708-AntiParvalbumin-antibody-3C9-IHC-rat-cerebellum.JPG "Anti-Parvalbumin antibody [3C9] (ab277625)")
Key features and details
- Mouse monoclonal [3C9] to Parvalbumin
- Suitable for: IHC-FrFl, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Parvalbumin antibody [3C9]
See all Parvalbumin primary antibodies -
Description
Mouse monoclonal [3C9] to Parvalbumin -
Host species
Mouse -
Tested applications
Suitable for: IHC-FrFl, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Parvalbumin.
Database link: P20472 -
Positive control
- WB: Mouse muscle, full length human parvalbumin. IHC-FrFl: Rat cerebellum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
3C9 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry - Anti-Parvalbumin antibody [3C9] (ab277625)
Perfusion-fixed, free floating sections of rat cerebellum tissue stained for parvalbumin using ab277625 (green) at 1/1000 dilution counterstained with chicken pAb to calbindin (red) at 1/2000 dilution in immunohistochemical analysis. The blue DAPI is staining for nuclear DNA. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear green.
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![Western blot - Anti-Parvalbumin antibody [3C9] (ab277625)](https://www.abcam.com/ps/products/277/ab277625/Images/ab277625-432707-AntiParvalbumin-antibody-3C9-WB-mouse-muscle-Full-length-human-parvalbumin.JPG)
Western blot - Anti-Parvalbumin antibody [3C9] (ab277625)Lanes 2-5 : Anti-Parvalbumin antibody [3C9] (ab277625) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Mouse muscle lysate
Lane 3 : Full length His-tagged recombinant human parvalbumin
Lane 4 : Full length human calretinin
Lane 5 : Full length human calbindin
Predicted band size: 12 kDa
