LDH Assay Kit (Cytotoxicity) (ab65391)
Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
LDH Assay Kit (Cytotoxicity)
See all Lactate Dehydrogenase kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Assay time
1h 00m -
Product overview
For a more sensitive LDH Assay Kit to use as a cytotoxicity assay, we recommend ab65393.
LDH Assay Kit (Cytotoxicity) ab65391 provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells.
The assay takes ~0.5-1 hr. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm.
This kit was previously called LDH-Cytotoxicity Assay Kit I.
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Notes
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane.
If you would like to use a fluorometric reading, please refer to LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004).
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 400 tests Catalyst (Lyophilized) 1 vial Dye Solution 1 x 45ml -
Research areas
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all
Images
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Functional studies - ab65391 LDH-Cytotoxicity Assay Kit (400 assays)
LDH release measured at various cell amounts
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Functional Studies - Lactate Dehydrogenase-Cytotoxicity Assay Kit (ab65391)Jurkat cells were cultured in a 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium according to the kit instructions. Light bar: Low control; Dark bar: High control.
