Mouse DLL1 ELISA Kit (ab208342)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse DLL1 ELISA Kit
See all DLL1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse serum 5 2.8% Inter-assay Sample n Mean SD CV% Mouse serum 3 7.2% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 94% - 104% Serum 100 99% - 101% Hep Plasma 101 98% - 104% EDTA Plasma 103 102% - 105% Cit plasma 104 103% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse DLL1 ELISA Kit (ab208342) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DLL1 protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse DLL1 with 24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
DLL1 (Delta-like protein 1) is a transmembrane glycoprotein that is needed during development for cell-to-cell communication in embryos and in the nervous system development. In adult tissues, DLL1 is involved in morphogenesis and remodeling and is overexpressed in several types of cancers.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse DLL1 Capture Antibody 1 x 600µl 10X Mouse DLL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse DLL1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. -
Tissue specificity
Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. -
Sequence similarities
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
Post-translational
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Delta
- Delta (Drosophila) like 1
- Delta like 1
see all -
Database links
- Entrez Gene: 13388 Mouse
- SwissProt: Q61483 Mouse
- Unigene: 4875 Mouse
Images
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Other - Mouse DLL1 ELISA Kit (ab208342)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse DLL1 standardBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native DLL1 in (mouse) serum and plasma samples.The concentrations of DLL1 were measured in duplicates, interpolated from the DLL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (heparin) 2.5% and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DLL1 concentration was determined to be 26 ng/mL in serum, 26 ng/mL in plasma (citrate), 34 ng/mL in plasma (heparin) and 29 ng/mL in plasma (EDTA).
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Sandwich ELISA - Mouse DLL1 ELISA Kit (ab208342)To learn more about the advantages of recombinant antibodies see here.
