Anti-CTF/NFIA antibody (ab228897)
Key features and details
- Rabbit polyclonal to CTF/NFIA
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CTF/NFIA antibody -
Description
Rabbit polyclonal to CTF/NFIA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-Fr MouseIHC-P MouseRatWB Human
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Immunogen
Recombinant fragment within Human CTF/NFIA (internal sequence). The exact sequence is proprietary.
Database link: Q12857 -
Positive control
- WB: HeLa nuclear lysate. IHC-P: Mouse liver and muscle tissues; Rat hindbrain tissue. IHC-Fr: Adult mouse retina tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CTF/NFIA antibody (ab228897)Anti-CTF/NFIA antibody (ab228897) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Frozen sections) - Anti-CTF/NFIA antibody (ab228897)Frozen section of adult mouse retina tissue stained for CTF/NFIA using ab228897 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-Tubulin 3/TUJ1 antibody (red) at 1/250 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-CTF/NFIA antibody (ab228897)4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CTF/NFIA (green) using ab228897 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (ab228897)Paraffin-embedded mouse liver tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (ab228897)Paraffin-embedded mouse muscle tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTF/NFIA antibody (ab228897)Paraffin-embedded rat hindbrain tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.