Anti-CaSR antibody (ab223360)
Key features and details
- Rabbit polyclonal to CaSR
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CaSR antibody
See all CaSR primary antibodies -
Description
Rabbit polyclonal to CaSR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat -
Immunogen
Synthetic peptide within Human CaSR aa 1-100 (N terminal). The exact sequence is proprietary. (NP_000379.2).
Database link: P41180 -
Positive control
- WB: Rat kidney lysate. ICC/IF: SK-N-BE cells.
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General notes
This product was previously labelled as Calcium Sensing Receptor
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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4% formaldehyde-fixed SK-N-BE cells stained for CaSR (green) using ab223360 at 1/100 dilution for 60 min at room temperature in ICC/IF.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223360 (D) Merge.
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Anti-CaSR antibody (ab223360) at 1/1000 dilution + Rat kidney lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/200 dilution
Predicted band size: 121 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Blocking buffer: 5% skim milk in TBST.
Primary incubation: 16 hours at 4ºC.
Color Development: TMB.