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Anti-CaSR antibody (ab223360)

Anti-CaSR antibody (ab223360)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CaSR
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CaSR antibody
    See all CaSR primary antibodies
  • Description

    Rabbit polyclonal to CaSR
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CaSR aa 1-100 (N terminal). The exact sequence is proprietary. (NP_000379.2).
    Database link: P41180

  • Positive control

    • WB: Rat kidney lysate. ICC/IF: SK-N-BE cells.
  • General notes

     This product was previously labelled as Calcium Sensing Receptor

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (ab223360)
    Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (ab223360)

    4% formaldehyde-fixed SK-N-BE cells stained for CaSR (green) using ab223360 at 1/100 dilution for 60 min at room temperature in ICC/IF.

    (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223360 (D) Merge.

     

  • Western blot - Anti-CaSR antibody (ab223360)
    Western blot - Anti-CaSR antibody (ab223360)
    Anti-CaSR antibody (ab223360) at 1/1000 dilution + Rat kidney lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/200 dilution

    Predicted band size: 121 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% skim milk in TBST.

    Primary incubation: 16 hours at 4ºC.

    Color Development: TMB.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CaSR antibody (ab223360)

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    Applications: Flow Cyt, IHC-Fr, IHC-P

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    Anti-CaSR antibody - N-terminal (ab219182)

    Applications: IHC-P

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    Anti-CaSR (phospho T888) antibody (ab62214)

    Applications: ICC/IF, IHC-P

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    Anti-CaSR antibody (ab137408)

    Applications: ICC/IF, IHC-P, WB

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    Anti-CaSR antibody - N-terminal (ab231447)

    Applications: IHC-P

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    Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)

    Applications: IHC-P

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    Applications: WB

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    Applications: IHC-P

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