Anti-CaSR (phospho T888) antibody (ab62214)
Key features and details
- Rabbit polyclonal to CaSR (phospho T888)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CaSR (phospho T888) antibody
See all CaSR primary antibodies -
Description
Rabbit polyclonal to CaSR (phospho T888) -
Host species
Rabbit -
Specificity
ab62214 antibody detects endogenous levels of CaSR only when phosphorylated at threonine 888.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic peptide corresponding to Human CaSR.
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General notes
This product was previously labelled as Calcium Sensing Receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab62214 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against the non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CaSR (phospho T888) antibody (ab62214)
Immunofluorescence analysis of HeLa cells, using ab62214 (1/500 - 1/1000) staining CaSR; in the presence and absence of immunizing peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR (phospho T888) antibody (ab62214)Ab62214 staining human normal renal medulla. Staining is localised to the cell membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
