Anti-CRHR2 antibody (ab104368)
Key features and details
- Rabbit polyclonal to CRHR2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-CRHR2 antibody
See all CRHR2 primary antibodies -
Description
Rabbit polyclonal to CRHR2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide corresponding to Rat CRHR2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125890) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.
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General notes
This product was previously labelled as Corticotropin Releasing Factor Receptor 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatAll applications HorseHamsterCowDogHumanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).Target
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Function
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 521831 Cow
- Entrez Gene: 482384 Dog
- Entrez Gene: 100055029 Horse
- Entrez Gene: 1395 Human
- Entrez Gene: 12922 Mouse
- Entrez Gene: 64680 Rat
- Omim: 602034 Human
- SwissProt: Q13324 Human
see all -
Alternative names
- corticotropin releasing factor receptor antibody
- Corticotropin releasing hormone receptor 2 antibody
- Corticotropin-releasing factor receptor 2 antibody
see all
Images
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Western blot - Anti-CRHR2 antibody (ab104368)All lanes : Anti-CRHR2 antibody (ab104368) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain
Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 51 + 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsCRHR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.
Datasheets and documents
References (0)
ab104368 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-CRHR2 antibody (ab104368)All lanes : Anti-CRHR2 antibody (ab104368) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain
Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 51 + 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsCRHR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.
