Anti-GNS antibody [EPR8329(2)] (ab154177)
![Anti-GNS antibody [EPR8329(2)] (ab154177)](https://www.abcam.com/ps/products/154/ab154177/Images/ab154177-389432-anti-gns-antibody-epr83292-western-blot-human-knockout-lysate.jpg "Anti-GNS antibody [EPR8329(2)] (ab154177)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8329(2)] to GNS
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-GNS antibody [EPR8329(2)]
See all GNS primary antibodies -
Description
Rabbit monoclonal [EPR8329(2)] to GNS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide, corresponding to residues in Human GNS (UniProt: P15586).
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Positive control
- WB: HeLa, PC3, NCI-H460 and 293T cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8329(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)All lanes : Anti-GNS antibody [EPR8329(2)] (ab154177) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GNS knockout HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 62 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab154177 observed at 90 kDa. Red - loading control ab7291 observed at 50 kDa.
ab154177 Anti-GNS antibody [EPR8329(2)] was shown to specifically react with GNS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265495 (knockout cell lysate ab257975) was used. Wild-type and GNS knockout samples were subjected to SDS-PAGE. ab154177 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)](https://www.abcam.com/ps/products/154/ab154177/Images/ab154177-160109-anti-gns-antibody-epr83292-western-blot.jpg)
Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)All lanes : Anti-GNS antibody [EPR8329(2)] (ab154177) at 1/10000 dilution
Lane 1 : PC3 cell lysate
Lane 2 : NCI-H460 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 62 kDa
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![Anti-GNS antibody [EPR8329(2)] (ab154177)](https://www.abcam.com/ps/products/154/ab154177/Images/ab154177-3-benefits-of-recombinant-antibodies.png)
Anti-GNS antibody [EPR8329(2)] (ab154177)
