Rat TCS Antibody Purification Kit (ab270562)
Overview
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Product name
Rat TCS Antibody Purification Kit -
Product overview
Rat TCS Antibody Purification Kit (ab270562) is designed for the purification of rat IgG fractions from tissue culture supernatants. The Rat Resin has a very high affinity and specificity for rat IgG molecules. The binding strength of bovine IgG to the Rat Resin is negligible; therefore, it can be used to selectively recover rat IgG from TCS samples. The rat antibody to be purified should be in 10 to 50 mL of tissue culture supernatant. Up to 600 µg of antibody can be purified in each run.
This product is manufactured by Expedeon, an Abcam company. It was previously called AbSelect™ Rat TCS Antibody Purification System. Expedeon product code 842-0500 is the same as the 3 pack size of this product.
The method involves capture of the rat antibody on the Rat resin and removal of unwanted substances using a simple wash procedure. The purified product is eluted and neutralized. Rat TCS Antibody Purification Kit is not suitable for use with antibodies from other species.
Antibodies purified using the Rat TCS Antibody Purification Kit are fully compatible with Lightning-Link® Antibody Conjugation kits and our Oligonucleotide Conjugation Kit.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 3 tests ab274185 - AbSelect 3 x 400µl ab273959 - Additional Collecting Tubes 1 vial ab274298 - Binding Buffer (10X) 1 x 18ml ab274299 - Elution Buffer 1 x 1500µl ab274174 - Neutralizer Buffer 1 x 500µl ab273914 - Spin Cartridge / Collecting Tube Assemblies 1 unit ab273958 - Spin cartridge/ collecting tube assemblies 1 unit ab274173 - Wash Buffer 1 x 12ml
Images
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Purification of Rat IgG
Purification of rat IgG using ab270562.