Anti-NEK6 antibody [OTI2H7] (ab117986)
![Anti-NEK6 antibody [OTI2H7] (ab117986)](https://www.abcam.com/ps/products/117/ab117986/Images/ab117986-129868-anti-nek6-antibody-oti2h7-western-blot.jpg "Anti-NEK6 antibody [OTI2H7] (ab117986)")
Key features and details
- Mouse monoclonal [OTI2H7] to NEK6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NEK6 antibody [OTI2H7]
See all NEK6 primary antibodies -
Description
Mouse monoclonal [OTI2H7] to NEK6 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human NEK6 aa 1-350.
Database link: Q9HC98 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA. IHC-P: Human breast adenocarcinoma, kidney carcinoma, ovary, ovary adenocarcinoma, thyroid carcinoma and endometrium tissues.
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General notes
Clone OTI2H7 (formerly 2H7).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab117986 was purified from mouse ascites fluids by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI2H7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-NEK6 antibody [OTI2H7] (ab117986)All lanes : Anti-NEK6 antibody [OTI2H7] (ab117986) at 1/1000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203609) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)](https://www.abcam.com/ps/products/117/ab117986/Images/ab117986-129867-anti-nek6-antibody-oti2h7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)](https://www.abcam.com/ps/products/117/ab117986/Images/ab117986-129866-anti-nek6-antibody-oti2h7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)](https://www.abcam.com/ps/products/117/ab117986/Images/ab117986-129865-anti-nek6-antibody-oti2h7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)](https://www.abcam.com/ps/products/117/ab117986/Images/ab117986-129864-anti-nek6-antibody-oti2h7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)](https://www.abcam.com/ps/products/117/ab117986/Images/ab117986-129863-anti-nek6-antibody-oti2h7-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)](https://www.abcam.com/ps/products/117/ab117986/Images/ab117986-129862-anti-nek6-antibody-oti2h7-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
