Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI2H7] to NEK6
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-NEK6 antibody [OTI2H7]
See all NEK6 primary antibodies
Description

Mouse monoclonal [OTI2H7] to NEK6
Host species

Mouse
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein corresponding to Human NEK6 aa 1-350.
Database link: Q9HC98

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA. IHC-P: Human breast adenocarcinoma, kidney carcinoma, ovary, ovary adenocarcinoma, thyroid carcinoma and endometrium tissues.
General notes

Clone OTI2H7 (formerly 2H7).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein G purified
Purification notes

ab117986 was purified from mouse ascites fluids by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI2H7
Isotype

IgG1
Research areas

Western blot - Anti-NEK6 antibody [OTI2H7] (ab117986)

All lanes : Anti-NEK6 antibody [OTI2H7] (ab117986) at 1/1000 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 36 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203609)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK6 antibody [OTI2H7] (ab117986)

ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com