Anti-NEK6 antibody [EPR5283] (ab133494)
![Anti-NEK6 antibody [EPR5283] (ab133494)](https://www.abcam.com/ps/products/133/ab133494/Images/ab133494-389438-anti-nek6-antibody-epr5283-western-blot-human-knockout-lysate.jpg "Anti-NEK6 antibody [EPR5283] (ab133494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5283] to NEK6
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-NEK6 antibody [EPR5283]
See all NEK6 primary antibodies -
Description
Rabbit monoclonal [EPR5283] to NEK6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to residues in Human NEK6 (UniProt ID: Q9HC98).
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Positive control
- WB: HepG2, HeLa, 293T and HT1080 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5283 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NEK6 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 36 kDaLanes 1-3: Merged signal (red and green). Green - ab133494 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.
ab133494 Anti-NEK6 antibody [EPR5283] was shown to specifically react with NEK6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265910 (knockout cell lysate ab258072) was used. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. ab133494 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)](https://www.abcam.com/ps/products/133/ab133494/Images/ab133494-147154-anti-nek6-antibody-epr5283-western-blot.jpg)
Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
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![Anti-NEK6 antibody [EPR5283] (ab133494)](https://www.abcam.com/ps/products/133/ab133494/Images/ab133494-3-benefits-of-recombinant-antibodies.png)
Anti-NEK6 antibody [EPR5283] (ab133494)
