Anti-ASH1L antibody [ASH5H03] (ab50981)
![Anti-ASH1L antibody [ASH5H03] (ab50981)](https://www.abcam.com/ps/products/50/ab50981/Images/ab50981-62786-anti-ash1l-antibody-ash5h03-western-blot.gif "Anti-ASH1L antibody [ASH5H03] (ab50981)")
Key features and details
- Mouse monoclonal [ASH5H03] to ASH1L
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-ASH1L antibody [ASH5H03] -
Description
Mouse monoclonal [ASH5H03] to ASH1L -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Human
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Immunogen
Recombinant fragment corresponding to Human ASH1L aa 1611-1728.
Sequence:IGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLNRLHRKESLPSNERAVQTL AGSQPTSDKPSQRPSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASG DDSVDSLLQRMVQNEDQE
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General notes
Previously labelled as KMT2H / ASH1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50981 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum -
Clonality
Monoclonal -
Clone number
ASH5H03 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-ASH1L antibody [ASH5H03] (ab50981)Anti-ASH1L antibody [ASH5H03] (ab50981) + Recombinant ASH1L (immunogen)
Predicted band size: 330 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.