Anti-SPOCK1 antibody (ab229935)
Key features and details
- Rabbit polyclonal to SPOCK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SPOCK1 antibody
See all SPOCK1 primary antibodies -
Description
Rabbit polyclonal to SPOCK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human SPOCK1 aa 250 to the C-terminus.
Database link: Q08629 -
Positive control
- WB: Mouse brain tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPOCK1 antibody (ab229935)Anti-SPOCK1 antibody (ab229935) at 1/2000 dilution + Mouse brain tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCK1 antibody (ab229935)Paraffin-embedded human kidney tissue stained for SPOCK1 using ab229935 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SPOCK1 antibody (ab229935)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SPOCK1 (green) using ab229935 at 1/100 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat anti-Rabbit IgG (H+L) as secondary antibody.
