Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15416] to Meis homeobox 3/MEIS3
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Meis homeobox 3/MEIS3 antibody [EPR15416]
See all Meis homeobox 3/MEIS3 primary antibodies
Description

Rabbit monoclonal [EPR15416] to Meis homeobox 3/MEIS3
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF7, HepG2, Human fetal lung and heart, rat brain, rat heart, rat kidney, rat spleen, RAW 264.7 and NIH 3T3 cell lysates; HeLa cells.
General notes
This product was previously labelled as Meis homeobox 3

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15416
Isotype

IgG
Research areas

Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961)

All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa
Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961)

All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution

Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa
Western blot - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961)

All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa
Flow Cytometry - Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961)

Flow Cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Meis homeobox 3/MEIS3 with ab185961 at 1/210 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Detection utilised Goat anti rabbit IgG (FITC) at 1/150 dilution.
Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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