Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15416] to Meis homeobox 3/MEIS3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Meis homeobox 3/MEIS3 antibody [EPR15416]
See all Meis homeobox 3/MEIS3 primary antibodies -
Description
Rabbit monoclonal [EPR15416] to Meis homeobox 3/MEIS3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB RatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF7, HepG2, Human fetal lung and heart, rat brain, rat heart, rat kidney, rat spleen, RAW 264.7 and NIH 3T3 cell lysates; HeLa cells.
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General notes
This product was previously labelled as Meis homeobox 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15416 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
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All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 41 kDa
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All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
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Flow Cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Meis homeobox 3/MEIS3 with ab185961 at 1/210 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Detection utilised Goat anti rabbit IgG (FITC) at 1/150 dilution.
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