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Anti-Engrailed and Invected antibody [4D9] (ab12454)

Key features and details

  • Mouse monoclonal [4D9] to Engrailed and Invected
  • Suitable for: IHC - Wholemount
  • Reacts with: Drosophila melanogaster
  • Isotype: IgG1

Overview

  • Product name

    Anti-Engrailed and Invected antibody [4D9]

  • Description

    Mouse monoclonal [4D9] to Engrailed and Invected

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC - Wholemountmore details

  • Species reactivity

    Reacts with: Drosophila melanogaster
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish, CrayfishDoes not react with: Mouse

  • Immunogen

    Recombinant fragment corresponding to Drosophila melanogaster Engrailed and Invected (C terminal).

  • Epitope

    Epitope has been localized to residues 38-58 of the homeodomain.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    4D9

  • Myeloma

    NS1

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)
    IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454) This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

    ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available here.

    Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.

  • IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)
    IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454) This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

    ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available here.

    Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining.

  • IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)
    IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454) This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

    ab12454 used in immunohistochemistry (Wholemount) at 1/200 dilution on Drosophila embryos. The protocol used is available here.

    Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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