Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSF-1-R antibody [YEYE311] (ab271294)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [YEYE311] to CSF-1-R
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CSF-1-R antibody [YEYE311]
    See all CSF-1-R primary antibodies

  • Description

    Rat monoclonal [YEYE311] to CSF-1-R

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 and J774A.1 whole cell lysate. IHC-P: Mouse liver and lymph node tissue. ICC/IF: RAW 264.7 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CSF-1-R antibody [YEYE311] (ab271294)
    Western blot - Anti-CSF-1-R antibody [YEYE311] (ab271294)
    All lanes : Anti-CSF-1-R antibody [YEYE311] (ab271294) at 1.208 µg/ml

    Lane 1 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 108 kDa


    Exposure time: 3 minutes


    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:14673177).
    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CSF1R with ab271294 at 1/500 dilution (2.416µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the Kupffer cells in the mouse liver. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)

    Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue labeling CSF1R with ab271294 at 1/500 (2.416µg/ml) dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the macrophages in mouse lymph node. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [YEYE311] (ab271294)
    Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [YEYE311] (ab271294)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling CSF1R with ab271294 at 1/50 dilution (24.16µg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in RAW 264.7 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab271294 at a 1/50 dilution (24.16µg/ml) followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Anti-CSF-1-R antibody [YEYE311] (ab271294)
    Anti-CSF-1-R antibody [YEYE311] (ab271294)

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