Anti-CSF-1-R antibody [YEYE311] (ab271294)
![Anti-CSF-1-R antibody [YEYE311] (ab271294)](https://www.abcam.com/ps/products/271/ab271294/Images/ab271294-394911-anticsf1r-antibody-yeye311-western-blot-raw2647.jpg "Anti-CSF-1-R antibody [YEYE311] (ab271294)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [YEYE311] to CSF-1-R
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse
Overview
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Product name
Anti-CSF-1-R antibody [YEYE311]
See all CSF-1-R primary antibodies -
Description
Rat monoclonal [YEYE311] to CSF-1-R -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P MouseWB Mouse
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW 264.7 and J774A.1 whole cell lysate. IHC-P: Mouse liver and lymph node tissue. ICC/IF: RAW 264.7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YEYE311 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CSF-1-R antibody [YEYE311] (ab271294)All lanes : Anti-CSF-1-R antibody [YEYE311] (ab271294) at 1.208 µg/ml
Lane 1 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 108 kDa
Exposure time: 3 minutesThe expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:14673177).
This blot was developed using a higher sensitivity ECL substrate.Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)](https://www.abcam.com/ps/products/271/ab271294/Images/ab271294-1-anticsf1r-antibody-yeye311-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CSF1R with ab271294 at 1/500 dilution (2.416µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the Kupffer cells in the mouse liver. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)](https://www.abcam.com/ps/products/271/ab271294/Images/ab271294-2-anticsf1r-antibody-yeye311-immunohistochemistry-mouse-lymph-node.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] (ab271294)Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue labeling CSF1R with ab271294 at 1/500 (2.416µg/ml) dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the macrophages in mouse lymph node. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [YEYE311] (ab271294)](https://www.abcam.com/ps/products/271/ab271294/Images/ab271294-3-anticsf1r-antibody-yeye311-immunocytochemistry-raw-2647.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [YEYE311] (ab271294)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling CSF1R with ab271294 at 1/50 dilution (24.16µg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in RAW 264.7 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab271294 at a 1/50 dilution (24.16µg/ml) followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
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![Anti-CSF-1-R antibody [YEYE311] (ab271294)](https://www.abcam.com/ps/products/271/ab271294/Images/ab271294-5-benefits-of-recombinant-antibodies.png)
Anti-CSF-1-R antibody [YEYE311] (ab271294)
