Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

Key features and details

  • Rabbit monoclonal [EP14557] to RFC4 - C-terminal
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RFC4 antibody [EP14557] - C-terminal
    See all RFC4 primary antibodies

  • Description

    Rabbit monoclonal [EP14557] to RFC4 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293T, K562, C6 and PC12 cell lysates; MCF7 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP14557

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    All lanes : Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    Western blot - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    All lanes : Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate

    Lysates/proteins at 1/20 dilution per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    Immunofluorescence analysis of MCF7 cells labeling RFC4 using ab192021 at 1/100 dilution (7.4 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.

    For the 2 negative cotrol images,  ab192021 was used at 1/100 dilution, followed by Goat anti Mouse IgG (Alexa Fluor®594) at 1/500 dilution as secondary antibody.

  • Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)
    Immunoprecipitation - Anti-RFC4 antibody [EP14557] - C-terminal (ab192021)

    Western blot analysis of immunoprecipitation pellet from HeLa cell lysate labeling RFC4 with ab192021 at 1/50 dilution (lane 1) or PBS control (lane 2). Secondary staining was with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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