Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PON1 antibody [EPR2893] (ab126597)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2893] to PON1
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PON1 antibody [EPR2893]
    See all PON1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2893] to PON1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PON1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • IP: Human liver lysate. WB: Human mouse and rat plasma and liver lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-PON1 antibody [EPR2893] (ab126597)
    Western blot - Anti-PON1 antibody [EPR2893] (ab126597)
    All lanes : Anti-PON1 antibody [EPR2893] (ab126597) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human liver lysate
    Lane 3 : Mouse plasma lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Rat plasma lysate
    Lane 6 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40-45 kDa
    why is the actual band size different from the predicted?



    The expression profile and molecular weights observed are consistent with what have been described in the literatures (PMID: 17906223, 30262871).

  • Immunoprecipitation - Anti-PON1 antibody [EPR2893] (ab126597)
    Immunoprecipitation - Anti-PON1 antibody [EPR2893] (ab126597)

    Purified ab126597 at 1/20 dilution (0.5µg) immunoprecipitating PON1 in Human liver lysate.
    Lane 1 (input): Human liver lysate 10µg
    Lane 2 (+): ab126597 + Human liver lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126597 in Human liver lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 40-45 kDa

  • Anti-PON1 antibody [EPR2893] (ab126597)
    Anti-PON1 antibody [EPR2893] (ab126597)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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