Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PON1 antibody [EPR2892] (ab92466)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2892] to PON1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PON1 antibody [EPR2892]
    See all PON1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2892] to PON1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PON1 aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human plasma cell lysate and ovary carcinoma tissue lysate; IHC-P: Human liver tissue. Mouse liver, kidney, and colon tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-PON1 antibody [EPR2892] (ab92466)
    Western blot - Anti-PON1 antibody [EPR2892] (ab92466)
    All lanes : Anti-PON1 antibody [EPR2892] (ab92466) at 1/1000 dilution

    Lane 1 : Human plasma cell lysate
    Lane 2 : ovarian cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
    Lane 2 : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    ab92466, at 1/100 dilution, staining PON1 in formalin-fixed, paraffin-embedded Human liver tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    ab92466 showing negative staining in Skeletal muscle tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    ab92466 showing negative staining in Normal colon tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    ab92466 showing negative staining in Normal breast tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)

    ab92466 showing negative staining in Normal brain tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Anti-PON1 antibody [EPR2892] (ab92466)
    Anti-PON1 antibody [EPR2892] (ab92466)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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