Anti-CSF-1-R (phospho Y561) antibody (ab192810)
Key features and details
- Rabbit polyclonal to CSF-1-R (phospho Y561)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSF-1-R (phospho Y561) antibody
See all CSF-1-R primary antibodies -
Description
Rabbit polyclonal to CSF-1-R (phospho Y561) -
Host species
Rabbit -
Specificity
ab192810 detects endogenous levels of CSF-1-R only when phosphorylated at tyrosine 561.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human CSF-1-R (phospho Y561) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of tyrosine 561 (N-S-Y(p)-T-F). NP_001275634.1.
Database link: P07333 -
Positive control
- WB: HepG2 cell extracts treated with PMA. IHC-P: Human brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS (without Mg2+and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192810 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSF-1-R (phospho Y561) antibody (ab192810)All lanes : Anti-CSF-1-R (phospho Y561) antibody (ab192810) at 1/500 dilution
Lane 1 : HepG2 cell extracts treated with PMA
Lane 2 : HepG2 cell extracts treated with PMA with antigen specific peptide
Predicted band size: 108 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R (phospho Y561) antibody (ab192810)Immunohistochemical analysis of formalin fixed paraffin embedded Human brain tissue labeling CSF-1-R with ab192910 at 1/50 dilution. The image on the right was pre-incubated with blocking peptide.
