Antibodies, Proteins, Kits and Reagents for Life Science

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Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP218] to PTEN - C-terminal
Suitable for: Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-PTEN antibody [SP218] - C-terminal
See all PTEN primary antibodies
Description

Rabbit monoclonal [SP218] to PTEN - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysate (ab150035). Flow Cyt: A431 and MCF7 cells IHC-P: Human prostate adenocarcinoma and colon adenocarcinoma tissues.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP218
Isotype

IgG
Research areas

Flow Cytometry - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Flow Cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling PTEN with purified ab228466 at 1/240 dilution (1.01 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
Western blot - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Anti-PTEN antibody [SP218] - C-terminal (ab228466) at 1/400 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cells labeling PTEN with ab228466 at 1/200 dilution (green) compared with a negative control rabbit IgG (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Cancer cells from prostate adenocarcinoma (panel A) and colon adenocarcinoma (panel D) show total loss or diminished expression of PTEN when SP218 is used. In contrast, nuclear staining (panel B and C, arrows) in cancer cells is present for clones D4.3 and 138G6. Diffuse cytoplasmic and stromal staining are prominent for clones D4.3 and 138G6. But interestingly, the stromal cell components (fibroblasts, lymphocytes, and endothelial cells) are stained with similar intensities across all images. This suggests that there is non-specific staining in stromal and cancer area with clones D4.3 and 138G6 when compared with SP218.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228466 at 1/200 dilution in immunohistochemical analysis.
Anti-PTEN antibody [SP218] - C-terminal (ab228466)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com