Antibodies, Proteins, Kits and Reagents for Life Science

391 996 ₸ Product size

100 µl 391 996 ₸ 10 µl 160 819 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y184] to PTEN
Suitable for: WB
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-PTEN antibody [Y184]
See all PTEN primary antibodies
Description

Rabbit monoclonal [Y184] to PTEN
Host species

Rabbit
Specificity

A 42kDa band is seen for some samples in addition to 50-54kDa band- we do not know the specificity of this band. For example Rat kidney, heart, spleen have bands around 50kDa but rat PC-12 cells have single band at ~42kDa.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human PTEN aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available as ab157804)
Positive control
- WB: HAP1, MCF7 and HEK-293 cell lysates; Human brain lysate; Mouse primary bone marrow derived macrophage whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y184
Isotype

IgG
Research areas

Western blot - Anti-PTEN antibody [Y184] (ab32199)

Lanes 1 and 5: PTEN knockout HAP1 cell lysate (20 µg)
Lanes 2 and 6: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Green signal from target – ab32199 observed at 47 kDa
Lanes 3 and 4: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 5 and 6: Merged (red and green) signal

ab32199 was shown to specifically recognize PTEN in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab32199 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-PTEN antibody [Y184] (ab32199)

Lanes 1: PTEN knockout HAP1 cell lysate (20 µg)
Lanes 2: Wild-type HAP1 cell lysate (20 µg)
Green signal from target
Red signal from loading control – ab8245 observed at 37 kDa

This western blot image is a comparison between ab32199 and a competitor's top cited mouse monoclonal antibody.
Western blot - Anti-PTEN antibody [Y184] (ab32199)

All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

All lanes : Brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 54 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-PTEN antibody [Y184] (ab32199)

All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

Lane 1 : MCF7 whole cell lysate
Lane 2 : HEK293 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-PTEN antibody [Y184] (ab32199)

Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution (Unpurified) + MCF7 cell lysate

Predicted band size: 47 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Western blot - Anti-PTEN antibody [Y184] (ab32199) This image is courtesy of an anonymous Abreview

All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution (Unpurified)

All lanes : Mouse primary bone marrow derived macrophage whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Treatment:
Lane 1 - control siRNA 24 hours
Lane 2 - PTEN siRNA 24 hours
Lane 3 - control siRNA 48 hours
Lane 4 - PTEN siRNA 48 hours
See Abreview
Western blot - Anti-PTEN antibody [Y184] (ab32199) This image is courtesy of an anonymous Abreview.

Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution + RBL-2H3 whole cell lysate

Secondary
Goat Anti-rabbit HRP at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 47 kDa

See Abreview
Anti-PTEN antibody [Y184] (ab32199)

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