ab79156, at 1/20 dilution, staining PTEN in frozen human breast tumor tissue sections by Immunohistochemistry. The reddish brown staining in the cytoplasm is indicative of the presence of PTEN protein in these cells. The nuclei appear purple and are negative for antibody staining. Interstitial tissue is negative as well.

This image was generated using the ascites version of the product.

ab79156, at 1/20 dilution, staining PTEN in frozen human breast tumor tissue sections by Immunohistochemistry. The reddish brown staining in the cytoplasm is indicative of the presence of PTEN protein in these cells. The nuclei appear purple and are negative for antibody staining. Interstitial tissue is negative as well.

This image was generated using the ascites version of the product.

Overlay histogram showing MCF7 cells stained with ab79156 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79156, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.