Anti-CDKN2A/p19ARF antibody (ab80)
Key features and details
- Rabbit polyclonal to CDKN2A/p19ARF
- Suitable for: ICC, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CDKN2A/p19ARF antibody
See all CDKN2A/p19ARF primary antibodies -
Description
Rabbit polyclonal to CDKN2A/p19ARF -
Host species
Rabbit -
Specificity
On Western blot the antibody reveals IVT murine p19ARF and a band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab2659) -
General notes
A monoclonal alternative to this target is available (ab26696).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedPurification notes
The serum has been purified on a peptide-NHS-activated Sepharose column. Specific antibodies have been eluted with 100 mM glycine pH 2.5. The purified antibodies have been dialysed against PBS pH 7.4.Clonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab80 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC Use a concentration of 1 µg/ml. Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle). WB 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples. Target
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Relevance
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
- Unigene: 4733 Mouse
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Alternative names
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
Images
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Western blot - Anti-CDKN2A/p19ARF antibody (ab80)This image is courtesy of Charles SherrAll lanes : Anti-CDKN2A/p19ARF antibody (ab80) at 1 µg/ml
Lanes 1-2 : mouse embryonic fibroblast total cell extract.
Lane 3 : mouse embryonic fibroblast total cell extract, with the p19ARF gene knocked out as a negative control.
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDaWestern blot of primary Mouse Embryo Fibroplasts (MEFs) using ab80 at 1 µg/ml. (30 µg of protein from total cell extracts per lane.)
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Immunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80)Pictures were generously provided by Dr. David Bertwistleab80 antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+).
Cells grown on coverslips were fixed with a 1:1 mixture of methanol:acetone at -20C for 10 mins. After drying, rehydrating and blocking, the co
Protocols
Datasheets and documents
References (207)
ab80 has been referenced in 207 publications.
- Kotowski K et al. 3PO as a Selective Inhibitor of 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 in A375 Human Melanoma Cells. Anticancer Res 40:2613-2625 (2020). PubMed: 32366406
- Mikawa R et al. p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction. Biomolecules 10:N/A (2020). PubMed: 32192082
- Alameda JP et al. Premature aging and cancer development in transgenic mice lacking functional CYLD. Aging (Albany NY) 11:127-159 (2019). PubMed: 30631004
- Hay J et al. Collaboration of MYC and RUNX2 in lymphoma simulates T-cell receptor signaling and attenuates p53 pathway activity. J Cell Biochem 120:18332-18345 (2019). PubMed: 31257681
- Benkafadar N et al. ROS-Induced Activation of DNA Damage Responses Drives Senescence-Like State in Postmitotic Cochlear Cells: Implication for Hearing Preservation. Mol Neurobiol N/A:N/A (2019). PubMed: 30693443
Images
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Western blot - Anti-CDKN2A/p19ARF antibody (ab80) This image is courtesy of Charles SherrAll lanes : Anti-CDKN2A/p19ARF antibody (ab80) at 1 µg/ml
Lanes 1-2 : mouse embryonic fibroblast total cell extract.
Lane 3 : mouse embryonic fibroblast total cell extract, with the p19ARF gene knocked out as a negative control.
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDaWestern blot of primary Mouse Embryo Fibroplasts (MEFs) using ab80 at 1 µg/ml. (30 µg of protein from total cell extracts per lane.)
-
Immunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80) Pictures were generously provided by Dr. David Bertwistle
ab80 antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+).
Cells grown on coverslips were fixed with a 1:1 mixture of methanol:acetone at -20C for 10 mins. After drying, rehydrating and blocking, the co
