HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)
![HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)](https://www.abcam.com/ps/products/202/ab202225/Images/ab202225-248862-anti-cdkn2a-p19arf-antibody-5-c3-hrp-western-blot.jpg "HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)")
Key features and details
- HRP Rat monoclonal [5-C3] to CDKN2A/p19ARF
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-CDKN2A/p19ARF antibody [5-C3]
See all CDKN2A/p19ARF primary antibodies -
Description
HRP Rat monoclonal [5-C3] to CDKN2A/p19ARF -
Host species
Rat -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MEF-1 whole cell lystate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
5-C3 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Images
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Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225) at 1/5000 dilution + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202225 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
