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Cancer Cell cycle Cell cycle inhibitors Ink4

Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

Price and availability

288 134 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [5-C3] to CDKN2A/p19ARF
  • Suitable for: WB, IP, ICC/IF, IHC-FoFr
  • Reacts with: Mouse
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-CDKN2A/p19ARF antibody [5-C3]
    See all CDKN2A/p19ARF primary antibodies
  • Description

    Rat monoclonal [5-C3] to CDKN2A/p19ARF
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.

  • Positive control

    • 3T3 cell lystate positive control.
  • General notes

    The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section. 

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    5-C3
  • Myeloma

    Y3/Ag1.2.3
  • Isotype

    IgG2b
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Ink4
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • INK4a/Arf

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
    Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
    Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
    Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
    All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
    Lane 2 : MEF total cell lysate

    Secondary
    All lanes : anti-rat IgG-HRP

    Predicted band size: 17 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
    Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
    Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

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    Applications: ICC/IF, IHC-P, IP, WB

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    HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)

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