Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Key features and details
- Rat monoclonal [5-C3] to CDKN2A/p19ARF
- Suitable for: WB, IP, ICC/IF, IHC-FoFr
- Reacts with: Mouse
- Isotype: IgG2b
Overview
-
Product name
Anti-CDKN2A/p19ARF antibody [5-C3]
See all CDKN2A/p19ARF primary antibodies -
Description
Rat monoclonal [5-C3] to CDKN2A/p19ARF -
Host species
Rat -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Hamster, Human -
Immunogen
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.
-
Positive control
- 3T3 cell lystate positive control.
-
General notes
The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5-C3 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Images
-
Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
-
All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
Lane 2 : MEF total cell lysate
Secondary
All lanes : anti-rat IgG-HRP
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
-
Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.