Anti-CaMKII (phospho T305 + T306) antibody (ab277784)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal to CaMKII (phospho T305 + T306)
- Suitable for: ICC
- Reacts with: Human
Overview
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Product name
Anti-CaMKII (phospho T305 + T306) antibody
See all CaMKII primary antibodies -
Description
Rabbit recombinant multiclonal to CaMKII (phospho T305 + T306) -
Host species
Rabbit -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide aa 300-400 (phospho T305 + T306).
Database link: Q9UQM7 -
General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Isotype
IgG
Images
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Immunocytochemistry - Anti-CaMKII antibody (ab277784)
For immunofluorescence analysis SH-SY5Y cells were fixed and permeabilized for detection of endogenous CamKIIpT305/306 using Anti-CamKIIpT305/306 Recombinant Rabbit Multiclonal Antibody (ab277784, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of CamKIIpT305/306 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of CamKIIpT305/306 in the cytoplasm. Panel e) shows loss of signal by competition with the CamKIIpT305/306 peptide, demonstrating antibody specificity, and panel f) demonstrates no competition with the non-phospho peptide. Panel μ) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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Anti-CaMKII antibody (ab277784)