Recombinant staphylococcus aureus PVL F protein (Active) (ab230258)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
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Product name
Recombinant staphylococcus aureus PVL F protein (Active) -
Biological activity
PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukS-PV in a concentration range of 1-100 nM.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Staphylococcus aureus -
Sequence
AQHITPVSEKKVDDKITLYKTTATSDSDKLKISQILTFNFIKDKSYDKDT LILKAAGNIYSGYTKPNPKDTISSQFYWGSKYNISINSDSNDSVNVVDYA PKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNGSKSFSETINYKQESYR TSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEMFLGSRQSN LNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD RYTNFWNQLHWIGNNYKDENRATHTS -
Predicted molecular weight
36 kDa -
Amino acids
25 to 325 -
Additional sequence information
This product is the mature full length protein from aa 25 to 325. The signal peptide is not included.
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Preparation and Storage
- LukF-PV
- Panton-Valentine Leukocidin F subunit
- PVL F subunit
Images
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SDS-PAGE - Recombinant staphylococcus aureus PVL F protein (Active) (ab230258)
SDS-PAGE analysis of 1 µg (lane 1) and 5 µg (lane 2) ab230258.
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Western blot - Recombinant staphylococcus aureus PVL F protein (Active) (ab230258)Anti-PVL F polyclonal antibody at 0.5 µg/ml +Recombinant staphylococcus aureus PVL F protein (Active) (ab230258) at 0.1 µg
Secondary
Anti-rabbit IgG-HRP conjugateFollowed by TMB substrate.
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Functional Studies - Recombinant staphylococcus aureus PVL F protein (Active) (ab230258)Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of ab230258 and LukS-PV at equimolar concentration for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubating for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470/690 nm. EC50 values were found to be 0.907 nM.