Biotin Anti-Phosphoserine antibody (ab9335)
Key features and details
- Biotin Rabbit polyclonal to Phosphoserine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Phosphoserine antibody
See all Phosphoserine primary antibodies -
Description
Biotin Rabbit polyclonal to Phosphoserine -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. -
Tested applications
Suitable for: WB, IP, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
BSA and KLH-phosphoserine conjugates.
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Positive control
- Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.00
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with phosphoserine-agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-Phosphoserine antibody (ab9335)
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
