Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14612] to MS2
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-MS2 antibody [EPR14612]
See all MS2 primary antibodies
Description

Rabbit monoclonal [EPR14612] to MS2
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- THP1, KM3, Raji, C6, Raw264.7, PC12 and NIH 3T3 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14612
Isotype

IgG
Research areas

Western blot - Anti-MS2 antibody [EPR14612] (ab186432)

All lanes : Anti-MS2 antibody [EPR14612] (ab186432) at 1/20000 dilution

Lane 1 : THP1 cell lysate
Lane 2 : KM3 cell lysate
Lane 3 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Blocking buffer and concentration: 5% NFDM/TBST
Western blot - Anti-MS2 antibody [EPR14612] (ab186432)

All lanes : Anti-MS2 antibody [EPR14612] (ab186432) at 1/5000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Blocking buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-MS2 antibody [EPR14612] (ab186432)

Western blot analysis on immunoprecipitation pellet from Raji cell lysate, labeling MS2 immunoprecipitated using ab186432 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-MS2 antibody [EPR14612] (ab186432)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com