Human Cathepsin B ELISA Kit (ab119584)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Cathepsin B antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Cathepsin B antibody 1 x 130µl Lyophilized recombinant Human Cathepsin B standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- Amyloid precursor protein secretase
- APP secretase
- APPS
see all -
Database links
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
Images
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ELISA: Cathepsin B Human ELISA Kit (ab119584)
Cathepsin B measured in culture supernatants from control (-) or stimulated cells cells (P/L: 24 hours with 50 ng x mL-1 of PMA (ab120297) with 1 ug x mL-1 of LPS (Sigma) for the last 6 hours; PHA: 48 hours with 2% PHA-M; LifeTechnologies). Data shown represents duplicates (+/- SD), samples tested in the dilution range of 1/1-1/20.
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ELISA: Cathepsin B Human ELISA Kit (ab119584)Cathepsin B measured in biological fluids (tested in the range of 1/10-1/1000; duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve Using ab119584.