Anti-Phosphoserine antibody (ab9332)
Key features and details
- Rabbit polyclonal to Phosphoserine
- Suitable for: ELISA, IP, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phosphoserine antibody
See all Phosphoserine primary antibodies -
Description
Rabbit polyclonal to Phosphoserine -
Host species
Rabbit -
Specificity
Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data. -
Tested applications
Suitable for: ELISA, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to Phosphoserine. KLH-phosphoserine conjugate and phosvitin mixture.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.00
Preservative: 0.1% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phosphoserine antibody (ab9332) This image is courtesy of an Abreview submitted by Karin Birkenkamp-DemtroederAll lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)
All lanes : Whole cell lysate of monkey COS7 cells
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution
Blocking Step: 10% Milk for 1 hour at room temperature -
Western blot - Anti-Phosphoserine antibody (ab9332)Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
