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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-PTEN (phospho T366 + S370) antibody (ab226801)

Anti-PTEN (phospho T366 + S370) antibody (ab226801)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PTEN (phospho T366 + S370)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PTEN (phospho T366 + S370) antibody
    See all PTEN primary antibodies
  • Description

    Rabbit polyclonal to PTEN (phospho T366 + S370)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human PTEN (C terminal) (phospho T366 + S370). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: P60484

  • Positive control

    • ICC/IF: HeLa cells. WB: A431 whole cell extract.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Phosphatases
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • PTEN pathway
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Western blot - Anti-PTEN (phospho T366 + S370) antibody (ab226801)
    Western blot - Anti-PTEN (phospho T366 + S370) antibody (ab226801)
    All lanes : Anti-PTEN (phospho T366 + S370) antibody (ab226801) at 1/500 dilution

    Lane 1 : Untreated A431 (human epidermoid carcinoma cell line) whole cell extracts
    Lane 2 : CIP treated A431 (human epidermoid carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-PTEN (phospho T366 + S370) antibody (ab226801)
    Immunocytochemistry/ Immunofluorescence - Anti-PTEN (phospho T366 + S370) antibody (ab226801)

    4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PTEN (green) using ab226801 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PTEN (phospho T366 + S370) antibody (ab226801)

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