Anti-SHP1 (phospho Y536) antibody (ab131500)
Key features and details
- Rabbit polyclonal to SHP1 (phospho Y536)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHP1 (phospho Y536) antibody
See all SHP1 primary antibodies -
Description
Rabbit polyclonal to SHP1 (phospho Y536) -
Host species
Rabbit -
Specificity
ab131500 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human -
Immunogen
Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of tyrosine 536 (SEYpGN) of Human SHP1 (NP_002822.2).
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Positive control
- HeLa cells; Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131500 was purified by affinity chromatography using epitope specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling SHP1 (phospho Y536) with ab131500 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling SHP1 (phospho Y536) with ab131500 at 1/50 dilution (left) or ab131500 preincubated with blocking peptide (right).