WST-1 Assay Reagent - Cell Proliferation (ready to use) (ab155902)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
WST-1 Assay Reagent - Cell Proliferation (ready to use)
See all Cell viability/proliferation kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay time
0h 30m -
Product overview
WST-1 Assay Reagent ab155902 provides a simple, accurate and ready-to-use assay to measure cell proliferation, cell viability and cytotoxicity in mammalian cells.
The WST-1 assay protocol is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. The larger the number of viable cells, the higher the activity of the mitochondrial dehydrogenases, and in turn the greater the amount of formazan dye formed.
The WST-1 assay protocol is very simple:
- add the WST-1 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
- shake the plate to mix the contents
- analyze the amount of formazan dye produced by measuring the absorbance at 440 nmWe also offer complete WST-1 assay kits: WST-1 assay kit ab65473 which is the full kit version of this reagent, and WST-1 assay kit ab65475 which uses an alternative WST-1 analog.
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Notes
WST-1 assays are non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 2500 tests WST1 Reagent 1 x 2500 tests -
Research areas
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Relevance
Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. -
Alternative names
- Cell Cytotoxicity
- cell tracking
