XTT Assay Kit ab232856 provides an easy to use tool for use with cell cultures to study changes in the number of cells and their metabolic activity. This provides insights into cell viability, cell proliferation, and cytotoxicity.

The XTT assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.

Reduction of XTT during the assay produces a water-soluble orange-colored formazan product which dissolves directly into the culture medium, eliminating the need for an additional solubilization step (as is required in the MTT assay, but not in the MTS assay).

The formazan product can be detected using an absorbance-based microplate reader.

As the reagents used in the XTT and their products are non-toxic and the assay does not involve cell lysis, the XTT assay can be used to measure multiple timepoints or for continual measurement.

XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) is a tetrazolium-based compound like the reagents used in the MTT assay and the MTS assay.

Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.

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• Kits
• Cell Metabolism Kits
• Other Metabolism Assay

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• Kits
• Cell Metabolism Kits
• Cell Viability and Senescence Kits

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• Cell Damage Kits
• Cell viability, plasma membrane damage

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• Kits
• Cell Damage Kits
• proliferation and senescence