Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter (ab176726)
Key features and details
- Assay type: Cell-based (quantitative)
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter
See all Cell viability/proliferation kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) | Cytopainter (ab176726) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Ex/Em = 403/454 nm excited with a violet laser at Ex = 405 nm.
The CytoLabeling Blue Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.
Main features of CytoLabeling Blue Reagent:
- It eliminates the dye efflux drawback associated with CFSE
- It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
- It is retained up to 9 generations after staining
- Fluorescence is preserved upon formaldehyde fixation
This product can be used for imaging or flow cytometric analysis.
Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide
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Notes
Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests 1000 tests CytoLabeling Blue Reagent 1 vial 1 x 2 vials -
Research areas
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Relevance
Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. -
Alternative names
- Cell Cytotoxicity
- cell tracking
Images
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Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Blue Fluorescence (Ex-405nm) (ab176726)
Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Blue Fluorescence (Ex/Em = 403/454nm) (ab176726), CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (Ex/Em = 511/525nm) (ab176735) and CytoPainter Cell Proliferation Staining Staining Reagent - Red Fluorescence (Ex/Em = 628/643) (ab176736) in PBS buffer (pH=7.2).
