Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23448-119] to CRALBP - BSA and Azide free
  • Suitable for: IHC-Fr, Flow Cyt, IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free
    See all CRALBP primary antibodies

  • Description

    Rabbit monoclonal [EPR23448-119] to CRALBP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human eyeball tissue lysate; Mouse eyeball tissue and P20 retina lysates; Rat eyeball tissue lysate; SK-MEL-2 and SK-MEL-28 whole cell lysates. IHC-P: Rat retina tissue, Mouse retina tissue; Human retina tissue. IHC-Fr: Mouse retina tissue; Rat retina tissue. Flow Cyt: SK-MEL-28 cells. IP: SK-MEL-28 whole cell lysate.

  • General notes

    ab272171 is the carrier-free version of ab243664. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human retina. The section was incubated with ab243664 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Flow Cytometry - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Flow Cytometry - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SK-MEL-28 (Human malignant melanoma) cells labelling CRALBP with ab243664 at 1/700 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    CRALBP was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma), whole cell lysate with ab243664 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243664 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: SK-MEL-28 (human malignant melanoma), whole cell lysate 10 ug

    Lane 2: ab243664 IP in SK-MEL-28 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243664 in SK-MEL-28 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 100 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling CRALBP with ab243664 at 250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 dilution (Green). Positive staining on mouse retina especially on retinal pigment epithelial cell (RPE) and Muller cells. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat retina tissue labeling CRALBP with ab243664 at 250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 dilution (Green). Positive staining on rat retina especially on retinal pigment epithelial cell (RPE) and Muller cells. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human kidney. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243664).

  • Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)
    Anti-CRALBP antibody [EPR23448-119] - BSA and Azide free (ab272171)

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